Diagnosing tuberculosis (TB) early can allow patients to receive the medicine they need and also help prevent the disease from spreading. But in resource-limited areas, equipment requirements and long wait times for results are obstacles to diagnosis and treatment. To tackle this problem, scientists report in ACS Sensors the development of a fast, paper-based tuberculosis test that can be read with a smartphone.
The World Health Organization estimates that in 2015, 1.4 million people died from TB, with most of these deaths occurring in low- and middle-income countries. Early diagnosis could help curb these numbers. But conventional methods such as sputum smear microscopy, chest X-rays and molecular-based tests require equipment, electricity and specialized personnel that are not always available in remote or developing areas. So Chien-Fu Chen and colleagues set out to come up with a more practical diagnostic test that can be read with a smartphone, a technology that is increasingly available in emerging economies.
The researchers combined gold nanoparticles with fluorescent single-stranded DNA sequences that bind to the genetic material of Mycobacterium tuberculosis, the bacteria that cause TB. These nanoparticles were then incorporated into a paper-based device. Adding even a minute amount of lab-derived, double-stranded DNA from M. tuberculosis changed the color of the test spots within an hour. A smartphone camera was used to analyze the color change to determine the bacterial concentration. The researchers also tested a tissue sample from an infected patient to further demonstrate that the device could be used in the field.
- Tsung-Ting Tsai, Chia-Yu Huang, Chung-An Chen, Shu-Wei Shen, Mei-Chia Wang, Chao-Min Cheng, Chien-Fu Chen. Diagnosis of Tuberculosis Using Colorimetric Gold Nanoparticles on a Paper-Based Analytical Device. ACS Sensors, 2017; DOI: 10.1021/acssensors.7b00450